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Journal of Experimental and Integrative Medicine
Periodical of GESDAV
Journal Home Page		ISSN: 1309-4572 (Print)
ISSN: 2146-3298 (Online)
Language: [ English ]  

  » Volume 3, Issue 2
      pp. 81-169
  » Volume 3, Issue 1
      pp. 1-79

 

Open Access

Original Article



Influence of low temperature storage on the properties of human placenta extracts

Oleg A. Nardid, Denys N. Pogozhykh, Svetlana V. Repina, Svitlana L. Rozanova, Ievgeniia I. Naumenko, Ekaterina D. Rozanova.

A B S T R A C TR E F E R E N C E S 

Abstract
Objective: This research represents the results of comparative investigations of the composition and biological activity of aqueous-saline extracts, obtained from fresh and stored at –196ºC human placental tissues.
Methods: The studying of quantitative composition of proteins in human placenta extract (HPE) was performed with gel-chromatography method. Content of lipid peroxidation products and catalase activity in the extracts were evaluated using spectrophotometric assay. Biological activity of extracts was evaluated by studying the modifications of structural and functional parameters of erythrocytes after exposure with the extracts.
Results: Our researches have shown that properties of HPE from the stored at –196ºС tissues slightly differ from those of fresh tissues. In extract of frozen tissues the content of high-molecular proteins is relatively higher. Content of malonyldialdehyde in extracts of the tissues stored at –196ºC does not change. Catalase activity decreases. For evaluation of biological effect of the extract on erythrocytes we have analyzed the following structural and functional parameters of red blood cells: spontaneous hemolysis, osmotic, and low-pH resistances, which characterize mechanical properties and stability of cellular membranes; relative content of main forms of hemoglobin (oxy-, deoxy-, and met-Hb), which elucidate the functioning of biochemical systems; and cytosol microviscosity, which reflects structure-dynamical state, determined, first of all, by interaction of cytosol and components of cytoskeleton with membranes.
Conclusion: It was shown that distinctions between the effects of extracts on erythrocytes were mainly manifested at cellular membrane level, not affecting main vital parameters.

Key words: Biological activity; Erythrocytes; Low-temperature storage; Placental extract


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How to Cite this Article
Pubmed Style

Nardid OA, Pogozhykh DN, Repina SV, Rozanova SL, Naumenko II, Rozanova ED. Influence of low temperature storage on the properties of human placenta extracts. J Exp Integr Med. 2012; 2(3): 213-217. doi:10.5455/jeim.290512.or.030


Web Style

Nardid OA, Pogozhykh DN, Repina SV, Rozanova SL, Naumenko II, Rozanova ED. Influence of low temperature storage on the properties of human placenta extracts. www.scopemed.org/?mno=14865 [Access: May 25, 2013]. doi:10.5455/jeim.290512.or.030


AMA (American Medical Association) Style

Nardid OA, Pogozhykh DN, Repina SV, Rozanova SL, Naumenko II, Rozanova ED. Influence of low temperature storage on the properties of human placenta extracts. J Exp Integr Med. 2012; 2(3): 213-217. doi:10.5455/jeim.290512.or.030


Vancouver/ICMJE Style

Nardid OA, Pogozhykh DN, Repina SV, Rozanova SL, Naumenko II, Rozanova ED. Influence of low temperature storage on the properties of human placenta extracts. J Exp Integr Med. (2012), [cited May 25, 2013]; 2(3): 213-217. doi:10.5455/jeim.290512.or.030


Harvard Style

Nardid, O. A., Pogozhykh, D. N., Repina, S. V., Rozanova, S. L., Naumenko, I. I. & Rozanova, E. D. (2012) Influence of low temperature storage on the properties of human placenta extracts. J Exp Integr Med, 2 (3), 213-217. doi:10.5455/jeim.290512.or.030


Turabian Style

Nardid, Oleg A., Denys N. Pogozhykh, Svetlana V. Repina, Svitlana L. Rozanova, Ievgeniia I. Naumenko, and Ekaterina D. Rozanova. 2012. Influence of low temperature storage on the properties of human placenta extracts. Journal of Experimental and Integrative Medicine, 2 (3), 213-217. doi:10.5455/jeim.290512.or.030


Chicago Style

Nardid, Oleg A., Denys N. Pogozhykh, Svetlana V. Repina, Svitlana L. Rozanova, Ievgeniia I. Naumenko, and Ekaterina D. Rozanova. "Influence of low temperature storage on the properties of human placenta extracts." Journal of Experimental and Integrative Medicine 2 (2012), 213-217. doi:10.5455/jeim.290512.or.030


MLA (The Modern Language Association) Style

Nardid, Oleg A., Denys N. Pogozhykh, Svetlana V. Repina, Svitlana L. Rozanova, Ievgeniia I. Naumenko, and Ekaterina D. Rozanova. "Influence of low temperature storage on the properties of human placenta extracts." Journal of Experimental and Integrative Medicine 2.3 (2012), 213-217. Print. doi:10.5455/jeim.290512.or.030


APA (American Psychological Association) Style

Nardid, O. A., Pogozhykh, D. N., Repina, S. V., Rozanova, S. L., Naumenko, I. I. & Rozanova, E. D. (2012) Influence of low temperature storage on the properties of human placenta extracts. Journal of Experimental and Integrative Medicine, 2 (3), 213-217. doi:10.5455/jeim.290512.or.030


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