Development and Validation of First Order Derivative UV-Spectrophotometric Method for Determination of Brimonidine tartrate in Bulk and in Formulation

pritam jain, Rahul khatal, sanjay surana

Abstract
Objective: A simple, rapid, accurate and economical First order UV-derivative spectrophotometric method has been developed for estimation of brimonidine tartrate from bulk and pharmaceutical formulation.
Materials and methods: The λmax of brimonidine tartrate in methanol and water was found to be 247 nm. The same spectrum was derivatised in to first order derivative; showed maximum amplitude of the trough at 262 nm. The drug follows linearity in the concentration range 2-12 µg/ml with correlation coefficient value 0.998.
Results: The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 99.58 % was found in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels i.e., 80%, 100% and 120 %. The % recovery was found to be in the range 99.00%– 101.63%. The low values of % R.S.D. are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The % R.S.D. value less than 2 indicate that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts.
Conclusion: The above method was a rapid and cost-effective quality-control tool for routine analysis of brimonidine tartrate in bulk and in pharmaceutical dosage form.

Key words: Brimonidine tartrate(BT); First order derivativeUV, quantitative determination, methanol.